Suh-Jen Jane Tsai
Providence University, Taiwan
Title: The determination of resveratrol, polydatin and quercetin in grape, red wine and pomace and liquid chromatography tandem mass spectrometry
Biography
Biography: Suh-Jen Jane Tsai
Abstract
Trans-resveratrol (trans-3,5,4'-trihydroxystilbene, t-RSV) is common found in grape and red wine. t-RSV has significant effects on lipid metabolism, anti-inflammatory, antioxidant, free radical scavenging, anti-allergy and cardiovascular diseases. Besides t-RSV is also an effective cancer preventive medication, can effectively inhibit tumor growth, and cancer formation. Polydatin, also called piceid (3,5,4'-trihydroxystilbene-3-O-β-D-glucopyranoside) is a stilbenoid glucoside and is a natural precursor of resveratrol. It can be found in the bark of Picea sitchensis. It can also be isolated from the Japanese Knotweed. Trans-polydatin is known to induce β-defensin production and reduce inflammatory response. Quercetin, (2-(3,4-dihydroxyphenyl) -3,5,7-trihydroxy-4H-chromen-4-one), is a flavanol, that is commonly found along with resveratrol in grapes and red wine. In nature, resveratrol and piceid exist in both trans- and cis-isomeric forms, which may have different biological effects due to their diverse chemical structures. Trans-isomer is biologically active while cis-isomer has less effect. However, trans-forms may be converted to cis-forms with the exposure to UV irradiation. LC-MS/MS has been one of the most popular instruments for the determination of various components in a diversity of samples. The effective pretreatments for grape, redwine and pomace (also called marc) were established for getting reliable results. Important analytical parameters were also studied. The reaction mechanisms involved in the LC-MS/MS determination were studied. The precision, accuracy, detection limit and reproducibility of the proposed method were evaluated to validate the reliability of the proposed method.