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Alessandro La Mantia

Alessandro La Mantia

University of Camerino, Italy

Title: Effect of cocoa roasting temperature and processing on chocolate polyphenols content


Biography: Alessandro La Mantia


The health properties of cocoa have been known for millennia but, with the support of analytical chemistry and clinical studies, its real potential can be exploited. Among the reported beneficial effects, cocoa contributes to reduce blood pressure, glycemia, improve cognitive function. These effects have been attributed to a plethora of antioxidant compounds. Unfortunately, polyphenol content is reduced during cocoa fermentation, drying, roasting and the other phases of chocolate production. We studied the variation of polyphenols content during all the different phases of chocolate production, with a special emphasis on roasting (three different roasting temperatures: 79, 101 and 133°C). Three cocoa bean sample have been followed during all the process to produce chocolate by analyzing the total polyphenols content (Folin-Ciocalteu assay), the antioxidant power (FRAP assay), the epicatechin content (HPLC and GC/MS), and epicatechin mean degree of polymerization (phloroglucinol adducts method). Results showed a similar trend for total polyphenol content and antioxidant power with an unexpected bell-shaped curve: An increase followed by a decrease for the three different roasting temperatures. At the intermediate temperature (101°C), the higher polyphenol content was found after roasting originating an asymmetric bell-shaped curve. Epicatecin content had a trend similar to that of total polyphenol content but, interestingly, the mean degree of polymerization data has the opposite behavior with some deviation in the case of the highest temperature, probably due to epicatechin degradation. It seems that roasting is able to free epicatechin from oligomers that can be partially reformed during the following treatments.